PMC

(a) Typical brown macules on chest with lesions confluent in the center and reticulate pattern at the periphery. (b) Typical brown macules in reticulate pattern on back

(A-B) Macules and papules of velvety and desquamative surface, centrally confluent and with a peripheral reticulate pattern

(A) the examples of the lowland brown/black and red/black Metriorrhynchini pattern A. (B) the examples of the highland yellow/black mimetic pattern B; left Cautires sp. (Metriorrhynchini: Cautirina), middle Metanoeus pendleburyi Kleine (Metriorrhynchini: Metanoeina), right Plateros sp. (Lycinae: Platerodini). (C) the examples of the highland reticulate mimetic pattern C; the codes R1–R4 designate the respective species in Fig. A. (D–F) maps of Borneo; from above D, the distribution of the lowland brown/black pattern A; E, yellow/black pattern B; and C, the reticulate pattern C. (G–J) the examples of the Calochromini co-mimetics, from above G, red/black form of the pattern A, (H) brown/black form of the pattern A; (I) yellow/black pattern B, and (J) reticulate pattern C. The code R5 is used to show the phylogenetic position of Micronychus pardus in Fig. B. The maps were downloaded from Natural Earth server (https://www.naturalearthdata.com) and edited using Adobe Photoshop CS6 (https://www.adobe.com/products/photoshop.html).

Immunofluorescence images of dual-labeled cells reveal the coexistence of endogenous AtPex16p in peroxisomes and ER. A to O, Representative confocal optical sections of nontransformed Arabidopsis cells processed for microscopy via the on-slide procedure whereby formaldehyde-fixed and pectolyase-cellulase-digested cells were spread on microscope slides, membranes were permeabilized with Triton X-100, and then primary and secondary antibodies and/or Concanavalin A-Alexa 594 were added to the cells (rather than to fixed/permeabilized cells in microfuge tubes as was done for cells shown in ). A to C: A, Green Cy2 punctate (arrows) and reticular patterns (arrowheads; rabbit anti-AtPex16p-42 IgGs; 1:10, overnight). B, The same cells exhibit a Cy5 punctate peroxisomal pattern (mouse anti-catalase monoclonal IgG; 1:500, 1 h). C, Merged image shows colocalized (yellow) catalase and AtPex16p in peroxisomes. D to F: D, Different portion of the same population of cells revealed similar Cy2 punctate (arrows) and reticular patterns (arrowheads; rabbit PA anti-AtPex16p IgGs; 1:500, 1 h). E, Same cells with Cy5 peroxisomes (mouse anti-catalase IgGs; 1:500, 1 h). F, Merged image shows colocalized catalase and Atpex16p in peroxisomes. G to I: G, Different portion of the same population of cells (rabbit PA anti-AtPex16p IgGs; 1:500, 1 h). H, Same cells (mouse anti-BiP monoclonal antibodies; 1:500, 1 h). I, Merged image produced a yellow reticulate pattern indicative of ER localization of AtPex16p. J to L: J, Reticulate pattern (PA anti-AtPex16p IgGs; 1:500, 1 h). K, Reticulate pattern (Concanavalin A-Alexa 594; 1:500, 1 h). L, Merged image (yellow) indicative of the ER localization of AtPex16p. M to O. M, Reticulate pattern (mouse anti-BiP monoclonal antibodies; 1:500, 1 h). N, Reticulate pattern (Concanavalin A-Alexa 594; 1:500, 1 h). O, Merged image produced a yellow reticulate pattern demonstrating that both Concanavalin A and BiP are colocalized in the same ER compartment. Bar = 5 μm.